Bioo HistaStrip

The HistaStrip™ Test Kit is a novel visual colorimetric strip test for the determination of histamine in fresh fish, other seafood, fish meal, wine and milk. The HistaStrip Test Kit uses a colorimetric enzyme assay to rapidly detect histamine. The HistaStrip tests can detect unsafe levels of histamine in fish samples in only 4 minutes. The strips contain a pad impregnated with histamine-specific enzyme to measure the chemical reduction of a colored dye indicator. Sample is added to a well containing buffer and then the strip is dipped into the well for one minute. The strip is then removed from the well and allowed to incubate on the bench for three minutes to allow the color reaction to proceed to completion. The strip color change is then visually compared to a reference color card to determine the amount of histamine present in the sample.

Histamine is a contaminant sometimes found in seafood when improperly handled. Quality tuna has histamine levels below 50 ppm. High levels of histamine in seafood can cause scombroid poisoning. The amount of histamine can be determined using ELISA or LC-MS. However, these traditional methods require very expensive equipment and time consuming sample preparation procedures.