Bioo Scientific – MaxSignal® Fructose/Glucose Assay Kit

SKU: BO 1306-01
Supplier: Bioo Scientific (view all products)

  • High sensitivity and low detection limit: 0.8 mg/L (ppm) glucose
  • A rapid 30 minute, robust enzyme-based assay
  • High reproducibility
  • Flexible assay format

 

The MaxSignal® Fructose/Glucose Assay Kit uses a coupled enzymatic reaction to detect glucose and/or fructose in food samples: glucose is detected by phosphorylation with hexokinase to make glucose-6-phosphate (G-6-P). This compound is then used as a substrate by the enzyme glucose-6-phosphate dehydrogenase to convert NADP+ to NADPH. This conversion, measured at 340 nm, is proportional to the level of glucose in the sample. Fructose is detected in similar manner after it is specifically converted to glucose-6-phosphate (G-6-P) using the enzyme phosphoglucose isomerase (PGI).

The absorbance of each sample at 340 nm is measured using a spectrophotometer. The concentration of glucose in each sample is then directly determined from the change in absorbance at 340 nm within 30 minutes. The assay offers both a cuvette-based format and a microtiter plate format for high-throughput sample analysis.

This kit is manufactured to the international quality standard ISO 9001:2008. ISO CI#: SARA-2009-CA-0114-A