Bioo Scientific – MaxSignal® Nitrate/Nitrite Assay Kit

• High sensitivity and low detection limit: 0.02 ppm
• A rapid 45 minute, robust enzyme-based assay
• High reproducibility
• Flexible assay format

The MaxSignal^® Nitrate/Nitrite Assay Kit is a cuvette-based enzymatic assay for the determination of nitrate and/or nitrite across a variety of food samples. The MaxSignal Nitrate/Nitrite Assay Kit uses a coupled enzymatic reaction scheme to detect nitrate and/or nitrite in food samples: nitrate in the sample is converted by nitrate reductase enzyme to make nitrite. The nitrite then reacts with sulfanilamide and N-(1-naphthyl)-ethylene-diamine to form a red diazo dye. This dye, measured at 540 nm, is proportional to the level of nitrate and/or nitrite in the sample.

The absorbance of each sample at 540 nm is measured using a spectrophotometer. The concentration of nitrate /nitrite in each sample is then directly determined from the diazo dye absorbance at 540 nm within 45 minutes.