Bioo Scientific – MaxSignal® Urea Assay Kit

SKU: BO 1307-01
Supplier: Bioo Scientific (view all products)

  • High sensitivity and low detection limit: 0.6 ppm
  • A rapid 30 minute, robust enzyme-based assay
  • High reproducibility
  • Flexible assay format

The MaxSignal® Urea Assay Kit uses a coupled enzymatic reaction scheme to detect urea in food samples. The assay measures the concentration of urea using the urease enzyme, which converts urea to ammonia. The ammonia produced by this reaction is then used as a substrate by the enzyme glutamate dehydrogenase to make glutamate. In the process, NADH is stoichiometrically converted to NAD+. This conversion, measured at 340 nm, is proportional to the level of urea in the sample.

The absorbance of each sample at 340 nm is measured using a spectrophotometer. The concentration of urea in each sample is then directly determined from the change in NADH absorbance at 340 nm within 25 minutes.

The assay offers both a cuvette-based format and a microtiter plate format for high-throughput sample analysis.

This kit is manufactured to the international quality standard ISO 9001:2008. ISO CI#: SARA-2009-CA-0114-A .