Ngaio Diagnostics > Molecular Biology > CellCover – parallel storage of proteins, RNA, and DNA

CellCover – parallel storage of proteins, RNA, and DNA

SKU: AE - 800
Supplier: Anacyte Laboratories (view all products)

CellCover

CellCover is the only reagent that allows parallel storage of proteins, RNA, and DNA in their cellular context.

  • Maintain cellular shape integrity without chemical crosslinking.
  • Protect DNA, RNA and proteins, protein expression and gene expression status.
  • Instant stabilization in one fast step.
  • Compatible with staining (in CellCover).
  • Non-toxic, ready to use solution.
  • For use in human and animal cells and solid tissues, including tumors and cultured cells (adherent, suspension, spheroids).
  • Truly representative molecules for genomic analysis, transcriptome analysis, and proteomic analysis.
  • Virtually all downstream applications are compatible with cell fixation using CellCover, including single cell RNA sequencing, NGS, protein sequencing, and more.

Cells Treated with CellCover Maintain Their Native State.

How to use CellCover

CellCover stabilizes cells in suspension, biopsies, or tissue without cell lysis, keeping cells close to in vivo morphology. CellCover is compatible with morphological analyses and staining procedures, including immunocytochemistry, immunohistochemistry, and flow cytometry, among many other downstream applications.

A unique and special feature of CellCover is the ability to perform additional molecular analysis on cells that have already been analyzed on a morphological or molecular level: DNA (e.g. PCR), RNA (e.g. RNA seq or single cell RNA-seq) or protein (e.g. protein modification analysis, protein sequencing, or proteomic analysis) can be isolated for subsequent applications after initial immunolabeling, immunohistochemistry, immunocytochemistry, or flow cytometry analyses.

Application of CellCover is simple. Remove cell culture medium, apply CellCover and incubate for two minutes. Then proceed with your standard protocol.

CellCover can be used in the following applications (and many other!):

  • Batch and single cell analysis
  • Flow cytometry / FACS
  • Immunocytochemistry
  • Immunohistochemistry
  •  DNA Sequencing
  • FISH
  • Microarray
  • NGS
  • PCR
  • Protein Sequencing
  • RNA Sequencing (RNA Seq)
  • Single Cell RNA Sequencing
  • Northern Blotting
  • Western Blotting
  • Many more applications

Contact Ngaio for samples

For more information please visit https://www.anacyte.com/