Accelerate Your Research: Zymo Research Game-Changing NGS Library Prep Kits
The pace of Next-Generation Sequencing (NGS) research is constantly accelerating, but the tedious, multi-step process of library preparation remains a common bottleneck. Researchers are constantly searching for workflows that are faster, simpler, and more reliable, especially when dealing with high-throughput screening or precious, low-input samples.
Zymo Research International has positioned itself as a leader in molecular biology, tackling this challenge head-on with a portfolio of unique NGS library preparation kits. These products are engineered for maximum efficiency, minimum hands-on time, and compatibility with Illumina® platforms, enabling scientists to move from sample to sequence-ready library with unprecedented speed and simplicity. Here are some of the key Zymo Research NGS Library Prep products and the groundbreaking features that set them apart.
Key Product Highlight: The Microbiomics Masterpiece
The Quick-16S™ Plus NGS Library Prep Kit
For researchers studying the microbiome, the Quick-16S Plus NGS Library Prep Kit offers a dramatic leap forward. Traditional 16S rRNA gene sequencing protocols are often time-consuming, requiring multiple rounds of bead-based cleanup and normalization steps. Zymo’s solution rewrites the rulebook:
- Ultimate Speed: Achieves library preparation in as little as 30 minutes of hands-on time for up to 96 samples, a workflow that is up to 8x faster than conventional protocols.
- Normalization-Free: The kit utilizes a single, auto-normalizing qPCR/PCR step, eliminating the need for subsequent library quantification via TapeStation® or gel electrophoresis. Simply pool by equal volume.
- Automation Ready: Designed for high-throughput (HTP) applications, the streamlined, single-PCR step protocol is 100% automation ready, minimizing human error and maximizing efficiency.
This kit targets various variable regions of the 16S rRNA gene (V4, V3-V4, etc.), ensuring high phylogenetic coverage for both bacteria and archaea.
Key Product Highlight: Simplifying Epigenomics
The Zymo-Seq WGBS Library Kits (WGBS & Trio WGBS)
Whole-Genome Bisulfite Sequencing (WGBS) is the gold standard for mapping DNA methylation at single-base resolution, but the procedure is notoriously complex. Zymo Research offers multiple innovative solutions to simplify this epigenetic analysis:
The Zymo-Seq WGBS Library Kit: This kit utilizes tagmentation technology to consolidate several steps, allowing for complete Whole Genome Bisulfite (WGBS) library preparation in a single tube and under 4 hours. This simplified workflow reduces hands-on time and minimizes preparation bias for more accurate methylation calling.
- The Zymo-Seq Trio WGBS Library Kit: An all-inclusive kit that allows for the co-detection of genetic and epigenetic information (genome-wide DNA methylation and true-end fragment analysis) in a single experiment. It’s specially optimized for challenging, low-input samples like Cell-Free DNA (cfDNA) and FFPE-derived DNA, making it ideal for clinical and translational research.
By using a direct ligation-based protocol, the Trio kit preserves native DNA termini, ensuring the highest accuracy for methylation detection, even with highly fragmented samples.
Beyond the Genome
While the Quick-16S and WGBS lines are standouts, Zymo Research also offers simplified workflows for other applications:
- Transcriptomics: The Zymo-Seq RiboFree™ Universal cDNA Kit is an easy-to-use, automation-friendly solution designed to generate total RNA libraries (excluding rRNA) from virtually any species, including animals, plants, and microbes.
Zymo Research is committed to removing the technical barriers to advanced genomic analysis. By focusing on streamlined protocols, minimal hands-on time, and robust results, their NGS Library Prep Kits empower researchers to spend less time at the bench and more time interpreting meaningful data. To learn more about these kits and explore Zymo Research’s complete portfolio, visit their NGS Library Prep Kits page.